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. 2016 May 30;15(5):861–871. doi: 10.1111/acel.12495

Figure 4.

Figure 4

PGI 2 elevation stimulates the expression of APH‐1α/1β via the PKA/CREB and JNK/c‐Jun signaling pathways in cultured neuronal cells. n2a cells were treated with PGI 2 (10 μm) in the absence or presence of H89 (1 μm) (a, b, f) or SP600125 (5 μm) (c, g) cells for 48 h. In distinct experiments, n2a cells were transfected with CREB (d) or c‐Jun siRNA (e) before treating the cells with PGI 2 (10 μm) for 48 h. APH‐1α/1β mRNA and protein levels were determined by qRT–PCR and Western blots, respectively (a, c–e). Phosphorylated CREB and c‐Jun as well as total CREB and c‐Jun were detected by immunoblotting using specific antibodies (a–e). The production of sAPPα and sAPPβ was determined by Western blots (f, g). The production of Aβ1–42 was determined by Aβ1–42 ELISA kits (f, g). The data represent the means ± SE of three independent experiments. *P < 0.05 with respect to the vehicle‐treated or vector‐transfected control. # P < 0.05 compared to PGI 2‐treated alone.