Figure 6.

Intranasal insulin enhances neurogenesis in APP/PS1 mice. (A) Western blot analysis of doublecortin (DCX), a marker of neurogenesis, in hippocampal homogenates from WT‐veh, APP/PS1‐veh, and APP/PS1‐ins mice. (B) Densitometric quantification of the blots after normalization to GAPDH expression. Data represent mean ± SEM. ^# P < 0.05 vs. APP/PS1‐veh group, n = 11 per group, one‐way ANOVA, followed by Bonferroni's post hoc test. (C) Immunolabeling for DCX in the dentate gyrus. The number of DCX‐positive cells in the dentate gyrus of APP/PS1 mice was slightly lower than that of WT mice. The APP/PS1‐ins group had more DCX‐immunoreactive cells than APP/PS1‐veh mice. Arrow indicates soma. Arrowhead indicates extensions. Scale bar = 50 μm. N = 6 per group.