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. 2016 Sep 7;7:1386. doi: 10.3389/fmicb.2016.01386

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Copyright © 2016 Saunders, Sen, Wilkinson and Gatto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rates of FabH activity with branched substrates at 30°C and 10°C. (A) Rates of substrate hydrolysis and product formation with equal substrate concentrations (10 μM) at 30°C. IB-CoA is orange and its product purple; IV-CoA is black and its product red; 2MB-CoA is green and its product blue. Forward rates for the pre-k_cat equilibrium varied from 0.1 s⁻¹ to 10 s⁻¹ and are color coded from light to dark. LmFabH concentration was 1 μM. Ac-CoA substrate is not shown as it is off scale for the duration of the experiment; its product is gray (at 0 μM). (B) Rates of substrate hydrolysis and product formation with equal substrate concentrations (10 μM) at 10°C. IB-CoA is orange and its product purple; IV-CoA is black and its product red; 2MB-CoA is green and its product blue. Forward rates for the pre-k_cat equilibrium were set to 10 s⁻¹. LmFabH concentration was 1 μM.