Figure 5.

Activity staining of different isoinhibitor spots of CpPI 63 against HGPs. CpPI 63 (75 μg) was subjected to Iso electric focusing (IEF) under non-reducing conditions as described in materials and methods using pH 4–7, 11 cm strips. Second dimension of electrophoresis was executed using gelatin SDS-PAGE (18%). After electrophoresis, the gel was incubated with Triton-x-100 followed by HGPs as described in materials and methods and stained with CBB R250 to visualize the isoinhibitor spots of CpPI 63 active against HGPs. The gel shown here is a selective representative from three replicates of CpPI 63 purified from three independent experiments.