Table 1.
Summary of SD parameters
| Comparison Group | % | Latency, min | # of Events | Duration, min | Delta [K+]o, mM |
|---|---|---|---|---|---|
| CS young | 44 | Mdn = 40.0 | Mdn = 0.0 | Mdn = 0.0 | Mdn = 15.04 |
| IQR = 8.89, 40.0 | IQR = 0.0, 1.50 | IQR = 0.0, 2.89 | IQR = 11.39, 34.05 | ||
| CS old | 88 | Mdn = 7.46 | Mdn = 3.0 | Mdn = 5.42 | Mdn = 40.92 |
| IQR = 2.57, 31.50 | IQR = 1.25, 4.75* | IQR = 3.19, 7.96* | IQR = 12.71, 144.39 | ||
| CS | 67 | Mdn = 13.98 | Mdn = 1.0 | Mdn = 1.65 | Mdn = 29.28 |
| IQR = 7.46, 40.0 | IQR = 0.0, 4.0 | IQR = 0.0, 11.22 | IQR = 14.28, 46.45 | ||
| w1118 | 100 | Mdn = 3.23 | Mdn = 6.50 | Mdn = 16.66 | Mdn = 37.86 |
| IQR = 0.70, 10.84* | IQR = 3.75, 9.0* | IQR = 8.25, 27.52* | IQR = 24.31, 65.41 | ||
| forR | 100 | Mdn = 5.67 | Mdn = 4.50 | Mdn = 12.96 | Mdn = 45.72 |
| IQR = 2.67, 9.30 | IQR = 3.0, 7.0 | IQR = 7.22, 20.65 | IQR = 28.23, 109.63 | ||
| fors2 | 74 | Mdn = 17.89 | Mdn = 4.0 | Mdn = 5.74 | Mdn = 24.04 |
| IQR = 7.46, 32.90* | IQR = 1.0, 7.0 | IQR = 1.06, 10.26* | IQR = 18.63, 27.77* |
Quantification of SD parameters for all flies tested. Three experimental comparisons were made: 1) young Canton S (CS; 4–5 days old) compared with old CS flies (35–40 days old); 2) CS flies compared with w1118 mutants; 3) forR (high PKG activity) compared with fors2 (low levels of PKG activity) flies. Percent (%), percent of preparations that exhibited SD in each group. Latency was measured from the time point of ouabain application to the inflection point of the first ouabain-induced event. Duration of SD activity was measured from the inflection point of the first SD event to the time of recovery following the last SD event. Delta [K+]o, overall change in [K+]o, calculated by measuring the [K+]o at the end of the 40-min treatment period and comparing it with initial levels before ouabain injections.
Significant differences between the corresponding flies within the comparison group. Details of the statistical tests performed and individual P values are reported in results.