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. 2016 Sep 7;36(36):9407–9419. doi: 10.1523/JNEUROSCI.1246-16.2016

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Copyright © 2016 the authors 0270-6474/16/369407-13$15.00/0

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Figure 3. — Rest target genes are inappropriately expressed in Mrest^sbu29/+ embryos. RNA Whole-mount in situ hybridization at 24 h and 6 dpf for Rest target genes for Mrest^sbu29/+ and Zrest^sbu29/+. Ectopic expression (white bracket or arrow) is observed with probes for snap25a (A,B), snap25b (E,F), and syt4 (I,J) but not nfsa (M,N) or amph (Q,R) in the hindbrain of Mrest^sbu29/+ embryos at 24 hpf. Increase expression in Mrest^sbu29/+ observed at 6 dpf in with nsfa (O,P) and amph (S,T) but not snap25a (C,D), snap25b, or syt4 probes. OV, Otic vesicle; MB, midbrain.