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. 2016 Sep 7;36(36):9435–9445. doi: 10.1523/JNEUROSCI.1782-16.2016

Figure 1.

Figure 1.

GCaMP3 is exclusively expressed in Müller cells. A, Müller glial cells extend fine processes in the inner plexiform layer (IPL) and the outer plexiform layer (OPL) and endfeet that surround arterioles and capillaries in the three capillary layers: superficial (Sup), intermediate (Int), and deep (Deep). GCL, Ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. B, Colocalization of GCaMP3 (detected with anti-GFP) and cellular retinaldihyde binding protein (CRALBP), a Müller cell marker, demonstrates that GCaMP3 is exclusively expressed in Müller cells. Similar results were obtained from retinas from four mice. C, Schematic of the retina. D, Colocalization of GCaMP3 with cell markers for vascular cells (Isolectin B4), pericytes (anti-NG2), and astrocytes (anti-GFAP) reveal no GCaMP3 expression in these cell types. Similar results were obtained from 2 other retinas for each cell type. Scale bars, 20 μm. E, Schematic of the retina. F, Neuronal cell bodies in the ganglion cell layer and the outer nuclear layer show no GCaMP3 expression. Müller cell stalks, identified by CRALBP labeling, lie between the neuronal cell bodies. Similar results were obtained from three other retinas.