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. 2016 Sep 7;36(36):9435–9445. doi: 10.1523/JNEUROSCI.1782-16.2016

Figure 8.

Figure 8.

Calcium signaling in Müller cells is necessary to evoke capillary but not arteriole dilation. A, The diameter of intermediate layer capillaries was monitored. GCL, Ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; OPL, outer plexiform layer. B, C, In eyecups, mean responses from intermediate layer capillaries show that stimulation evoked dilations in control mice (Ctrl) but not in IP3R2−/− littermates (n = 13 vessels from 4 control eyecups, n = 15 vessels from 4 IP3R2−/− eyecups; unpaired t test, p = 0.015; control, 7.6 ± 3.0% vessel dilation; IP3R2−/−, −0.37 ± 1.1% vessel dilation). D, The diameter of arterioles was monitored. E, F, Stimulation also evoked arteriole dilation in control mice. This dilation was not diminished in IP3R2−/− littermates (n = 6 vessels from 3 control eyecups, n = 7 vessels from 3 IP3R2−/− eyecups; unpaired t test, p = 0.72; control, 6.0 ± 1.2% vessel dilation; IP3R2−/−, 6.7 ± 1.2% vessel dilation). G, H, Similar responses were observed in vivo. Mean responses from intermediate layer capillaries show stimulus-evoked dilations in control mice but not in IP3R2−/− littermates (n = 14 vessels from 5 control mice, n = 14 vessels from 5 IP3R2−/− mice; unpaired t test, p = 0.0092; control, 1.1 ± 0.39% vessel dilation; IP3R2−/−, −0.25 ± 0.29% vessel dilation). I, J, Stimulus-evoked arteriole dilation in arterioles of control mice was not diminished in IP3R2−/− littermates (n = 5 vessels from 5 control mice, n = 5 vessels from 5 IP3R2−/− mice; unpaired t test, p = 0.41; control, 6.2 ± 1.9% vessel dilation; IP3R2−/−, 8.1 ± 1.0% vessel dilation). *p < 0.05; **p < 0.01.