Figure 3.
Regulation of transgene expression in GFP-GluN2a+/tTA+ using Dox. A, Timeline for temporal regulation of transgene expression using Dox. B, Transgene expression in GFP-GluN2A+/tTA+ mice is tightly regulated using Dox. Representative coronal images containing BLA show no GFP-GluN2A transgene expression in GFP-GluN2A+/tTA+ mice that were On-Dox at 6 weeks (Bi) and 8 weeks (Biii), but Bii depicts robust transgene expression when Dox is removed from the mice diet for 2 weeks. Scale bars (left to right), 250 μm (20×), 100 μm (50×), 50 μm (100×).