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TUNEL-positive: that is, apoptotic nuclei were stained with green spot. (a) TUNEL staining demonstrated cisplatin-induced cell apoptosis in the nondrug treatment group, DMY treatment group, cisplatin treatment group, and cisplatin with DMY (500 mg/kg) treatment group. Scale bar, 50 μm. (b) The TUNEL-positive cells were quantified using Imagine J software at 200x magnification. Data are presented as mean ± SD. ∗ P < 0.05 versus cisplatin treatment group.
