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. 2016 Sep 7;6:32511. doi: 10.1038/srep32511

Figure 1. Workflow of the experimental strategy used in this study.

Figure 1

Plasma samples from six experimental groups were trypsin digested, labeled with isobaric tags, pooled and then purified and fractionated using SCX method. Quantitative proteomic analyses were simultaneously performed using ESI-nanoLC-MS/MS and MALDI-nanoLC-MS/MS and then obtained data were analyzed with three types of software: MaxQuant, ProteinScape and Proteome Discoverer. Only proteins identified by all software were found to have a differential accumulation level.