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. 2016 May 30;9(6):e33264. doi: 10.5812/jjm.33264

Figure 2. Identification of the NR8383-EC Cell Line Expressing the ESAT6-CFP10 Protein With Fluorescence and RT-PCR.

Figure 2.

The ESAT6-CFP10 was fused to the N terminal of GFP of the pEGFP-N1 expression vector. A, the expression of the fusion protein was detected with fluorescence signals, using a fluorescence microscope; 1, NR8383 transfected with the recombinant eukaryotic plasmid pEGFP-N1-ESAT6-CFP10; 2, NR8383 non-transfected with the recombinant eukaryotic plasmid pEGFP-N1-ESAT6-CFP10; B, the mRNA level of ESAT6-CFP10 was analyzed with RT-PCR using total RNA extracted from cells; lane 1, the gene amplified from the NR8383-EC cells; lane 2, the gene amplified from NR8383 cells transfected with pEGFP-N1 plasmid; Lane 3, the gene amplified from non-transfected NR8383 rat alveolar macrophages.