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SpsB point mutations found in the USA300 Δmcr cro/cI(Q16stop) SAUSA300_0352(K44A) mutanta
| Amino acid substitution | MIC of compound 103 (fold increase versus MIC for USA300 WT) |
|---|---|
| Y30C | 4 |
| Y30N | 4 |
| Y30D | 8 |
| Y30S | 4 |
| V49L | 4 |
| H68Y | 8 |
| H69L | 4 |
| V167D | 8 |
| V167F | 8 |
Resistant clones were selected from USA300 Δmcr cro/cI(Q16stop) SAUSA300_0352(K44A) (strain GNE0175) bacteria cultured on agar containing compound 103 at fourfold its MIC. The SpsB mutations were identified by sequencing PCR products.
