FIG 3 .

Fluorescent fusions with FtsZ and EzrA lose mid-cell localization in M5 cells. (a) Localization of FtsZ^WT-CFP in BCBAJ020 cells (top) and FtsZ^G193D-CFP in BCBRP005 cells (bottom). Phase-contrast (left) and epifluorescence (right) images are shown. (b) SIM images of EzrA-mCherry (red) and cell wall labeled with Van-FL (green) in BCBAJ012 cells expressing FtsZ^WT (left) and in BCBRP006 cells expressing FtsZ^G193D (right). In the presence of FtsZ^G193D, EzrA-mCherry fails to localize as a mid-cell ring. Instead, structures assemble on only one side of the cell (white box) or in Y patterns compatible with one-turn helical structures (asterisk). More elongated BCBRP006 cells show EzrA-mCherry localized toward the poles of the cell (white arrow). Scale bars: 1 µm.