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. 2016 Jul 1;15(9):2863–2876. doi: 10.1074/mcp.M115.057695

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 1. — Development of SRM assay for quantification of KLK10. Human recombinant KLK10 was digested by trypsin and subjected to LC-MS/MS, LC-PRM and LC-SRM analyses. Top three peptides were selected based on LC-MS/MS data-dependent acquisition. Top 10 transitions were selected based on LC-PRM experiments. LC-SRM calibration curves for the top three peptides allowed for the selection of a top proteotypic peptide ALQLPYR for quantification of KLK10. Square and diamond shapes denote concentrations below and above LODs, respectively.