Table I. Analysis of five CVF samples by SRM. Analysis revealed expression of seven different kallikreins in CVF, with KLK10 and KLK13 being the most abundant. Quantification of KLK5 and KLK8 in CVF by mass spectrometry is reported here for the first time. The samples were analyzed in duplicates and the coefficient of variation of the calculated concentration ranged from 0.2% to 12.7%.
| Kallikrein | Protein concentration (μg/ml) |
||||
|---|---|---|---|---|---|
| CVF1 | CVF 2 | CVF 3 | CVF 4 | CVF 5 | |
| KLK5 | – | – | 1.0 | 0.1 | |
| KLK6 | 3.8 | – | – | 0.1 | 1.1 |
| KLK7 | – | – | 29.0 | 4.5 | – |
| KLK8 | – | – | 0.4 | 0.4 | – |
| KLK10 | 0.4 | 2.8 | 7.4 | 1.7 | 4.0 |
| KLK11 | 0.3 | 0.5 | – | 1.0 | 1.2 |
| KLK13 | 0.5 | 1.3 | 2.4 | 1.0 | 0.4 |