Table I. Primer sequences used in qRT-PCR of the 20 candidate winter-responsive protein genes and VIGS of the three unknown protein genes.

Spot no.a	Forward primer	Reverse primer	Produce length (bp)
4	TGTGCAAATGTTGTGGCAG	GCAGGATCAGAGGTATCCGC	194
6	TTGTTGCCTGCTTCACCTTC	GGGACAAGTTCAGCACACAG	171
7/10	TCCGAGTAAGTCCTCAGCCT	TTGGTTGTCTTCCCCAGCAA	170
8/9	GCCAAGCAGCCAAGAAGAAG	GCGGTTCCCTATTCAGTGCT	216
11	GAGTAAGTCCTCAGCCTGGG	TGTAGAGCACGTAGGGCTTT	280
13/14	CGCCGAATACACCAGCTACA	TGGGAAGCTGCATCTGTTGA	334
15	ACATGACATGCAAATCCTACGT	AGAAGAACTCTCGCCATCAAA	100
16	CACCTGGGAACTCACCTGTC	ACCATGTCCCTCTCCTCCTC	217
17	TTACCCCTCGACCAACTCCA	GGGCGAGCAACTCAACATTC	139
18	GCTCCCAGATCTTCAGCGAG	CGGCCGTTCTTGATCTCCTT	253
19	CCCAGGCGTTGTTGTTGAC	CGGAGCTCAAGGTGAAGGAG	147
20	GAGGGAAGAGACGACAGCAG	TGATTTTCAGCGAGGGGACG	111
21	CTCCAAGCCATACGAAACCG	GGTGTTCTCCGGGTCCTTAA	226
23	CCACCTGTGCCTTTCCAGAT	GCTGCGAAGGGTTACAGACT	254
24	ATCTCAACACCAGTGACCGT	GGGATAACGAGTGGGTGGAT	241
3/27	GGAACAAACGGGGAGGTCAT	AGTCATGGCGGAGTGAAAGG	274
β-actin	GTTCCAATCTATGAGGGATACACGC	GAACCTCCACTGAGAACAACATTACC	422
V-20	CGTCCCCTCGCTGAAAATCA	GAGAAGGCGCTGCTGTAGAC	198
V-6	TGATGCAGAAGAACCCACCA	TGTGTGCTGAACTTGTCCCA	171
V-21	GCAGTGATCTTCCGTGGACT	CACAAGCTGGACAATGGCAG	191
V-PDS	TCACAAGTAGCAGCGTCCA	GGTTTACTGGGGCGTTCACT	211

^a Spot no.: the numbering corresponds to the 2-DE gels in Fig. 2. Spot no. and protein name correspondingly are the same as shown in Table II (except for β-actin and PDS).