Table II. Extracted oligosaccharides from infant feces. Neutral mass, composition given as Hex_HexNAc_Fuc_Neu5Ac, oligosaccharide type, volume, and retention time are provided based on nanoLC-MS and nanoLC-MS/MS analysis.

Neutral mass	Composition	Oligosaccharide type	Volume	Retention time (min)
750.291	2_2_0_0	HMO digest	279610	9.5
3377.240	6_6_4_2	Fucosylated and sialylated complex	196226	9.8
1829.690	4_5_1_0	Fucosylated complex/hybrid	37904	10.0
1464.560	3_4_1_0	Fucosylated complex	138251	10.1
1261.480	3_3_1_0	N-glycan digest	549487	10.8
1407.540	3_3_2_0	Fucosylated complex	137259	10.8
1407.540	3_3_2_0	Fucosylated complex	153055	11.5
1204.460	3_2_2_0	HMO digest	502558	11.7
896.3490	2_2_1_0	HMO digest	182085	11.8
1407.540	3_3_2_0	Fucosylated complex	49544	11.8
1439.530	5_3_0_0	Intact HMO	184152	12.0
1058.400	3_2_1_0	HMO digest	574538	12.1
1204.460	3_2_2_0	HMO digest	2103312	12.1
1220.450	4_2_1_0	Intact HMO	2381382	12.1
1772.670	4_4_2_0	Fucosylated complex/Hybrid	57362	12.1
2354.820	11_2_1_0	Fucosylated high mannose	212239	12.2
1569.590	4_3_2_0	Fucosylated complex/hybrid	200417	12.3
1843.630	10_1_0_0	N-glycan digest	106806	12.4
1521.580	3_5_0_0	Complex	100672	12.5
1115.420	3_3_0_0	HMO digest or complex/hybrid	985914	12.6
1261.480	3_3_1_0	N-glycan digest	231816	12.6
912.343	3_2_0_0	HMO digest	1891751	12.9
1058.400	3_2_1_0	HMO digest	4549122	12.9
1204.460	3_2_2_0	HMO digest	5749952	12.9
896.3490	2_2_1_0	HMO digest	123440	13.0
2178.830	4_6_2_0	Fucosylated complex	595334	13.0
3579.300	6_7_2_3	Fucosylated and sialylated complex	1059568	13.0
1667.640	3_5_1_0	Fucosylated complex	94540	13.2
1382.510	5_2_1_0	Intact HMO or fucosylated complex/hybrid	47879	13.3
896.349	2_2_1_0	HMO digest	596870	13.4
1261.480	3_3_1_0	N-glycan digest	240843	13.4
1407.540	3_3_2_0	Fucosylated complex	175285	13.4
547.211	2_1_0_0	HMO digest	881324	13.6
1058.400	3_2_1_0	HMO digest	3824270	13.6
3465.260	6_5_4_3	Fucosylated and sialylated complex	124225	13.6
1220.450	4_2_1_0	Intact HMO	17185486	13.7
1115.420	3_3_0_0	HMO digest or complex/hybrid	947184	14.3
1261.480	3_3_1_0	N-glycan digest	9057561	14.3
1058.400	3_2_1_0	HMO digest	3292608	14.4
1204.460	3_2_2_0	HMO digest	1285079	14.4
1569.590	4_3_2_0	Fucosylated complex/hybrid	314997	14.5
2428.900	5_5_2_1	Fucosylated and sialylated complex/hybrid	273097	14.5
547.211	2_1_0_0	HMO digest	884078	14.6
912.343	3_2_0_0	HMO digest	71884	14.6
1382.510	5_2_1_0	Intact HMO or fucosylated complex/hybrid	3820702	14.6
1991.740	5_5_1_0	Fucosylated complex/hybrid	83669	15.0
2210.820	6_6_0_0	Complex	209486	15.0
2194.820	5_6_1_0	Fucosylated complex	708236	15.2
1204.460	3_2_2_0	HMO digest	455992	15.9
1893.700	6_3_2_0	Fucosylated hybrid	114767	15.9
1204.460	3_2_2_0	HMO digest	615505	16.1
1934.720	5_4_2_0	Fucosylated complex/hybrid	157273	16.1
1569.590	4_3_2_0	Fucosylated complex/hybrid	6479052	16.4
1731.640	5_3_2_0	Intact HMO	250306	16.4
2908.100	4_6_5_1	Fucosylated and sialylated complex	614794	16.4
1626.610	4_4_1_0	Fucosylated complex/hybrid	989645	16.5
1261.480	3_3_1_0	N-glycan digest	758468	16.6
912.343	3_2_0_0	HMO digest	108600	16.7
1115.420	3_3_0_0	HMO digest or complex/hybrid	1471326	16.7
1480.550	4_4_0_0	Complex/Hybrid	963689	16.7
1843.630	10_1_0_0	N-glycan digest	321012	16.7
2073.800	3_7_1_0	Fucosylated complex	2077952	16.7
1261.480	3_3_1_0	N-glycan digest	535548	16.8
1756.670	3_4_3_0	Fucosylated complex	109769	16.8
1423.530	4_3_1_0	Intact HMO	9728194	16.9
1569.590	4_3_2_0	Fucosylated complex/hybrid	779149	16.9
1731.640	5_3_2_0	Intact HMO	628018	16.9
1893.700	6_3_2_0	Fucosylated hybrid	152929	17.0
693.269	2_1_1_0	HMO Digest	170774	17.2
4267.542	7_6_5_4	Fucosylated and sialylated complex	708277	17.2
4121.480	7_6_4_4	Fucosylated and sialylated complex	491182	17.3
1724.660	3_6_0_0	Complex	175583	17.6
1714.630	4_3_1_1	Fucosylated and sialylated complex	705623	17.7
2908.100	4_6_5_1	Fucosylated and sialylated complex	344617	17.8
2997.110	6_7_2_1	Fucosylated and sialylated complex	106195	17.8
1115.420	3_3_0_0	HMO digest or complex/hybrid	1280814	17.9
1626.610	4_4_1_0	Fucosylated complex/hybrid	137941	18.0
1731.640	5_3_2_0	Intact HMO	2250499	18.0
3306.240	7_7_5_0	Fucosylated complex	4112155	18.0
1519.530	8_1_0_0	N-glycan digest	133033	18.1
2046.720	10_2_0_0	High mannose	104831	18.4
1439.530	5_3_0_0	Intact HMO	1206590	18.5
1585.590	5_3_1_0	Intact HMO	9652349	18.5
1683.630	4_5_0_0	Complex/Hybrid	900577	18.5
1074.400	4_2_0_0	Intact HMO	736506	18.6
547.211	2_1_0_0	HMO digest	295271	18.8
1058.400	3_2_1_0	HMO digest	285071	18.8
2354.820	11_2_1_0	Fucosylated high mannose	733722	18.8
1731.640	5_3_2_0	Intact HMO	6499774	19.0
1626.610	4_4_1_0	Fucosylated complex/hybrid	317167	19.2
2063.770	4_4_2_1	Fucosylated and sialylated complex	82428	19.2
2080.780	5_4_3_0	Fucosylated complex/hybrid	221900	19.4
2242.830	6_4_3_0	Intact HMO	1995646	19.4
1033.370	5_1_0_0	N-glycan digest	65705	19.6
2354.820	11_2_1_0	Fucosylated high mannose	253745	19.8
1074.400	4_2_0_0	Intact HMO	133408	20.0
1115.420	3_3_0_0	HMO Digest or complex/hybrid	863030	20.0
1569.590	4_3_2_0	Fucosylated complex/hybrid	522844	20.1
2242.830	6_4_3_0	Intact HMO	596267	20.1
2137.800	5_5_2_0	Fucosylated complex/hybrid	354307	20.3
1407.540	3_3_2_0	Fucosylated complex	352279	20.4
1439.530	5_3_0_0	Intact HMO	4727694	20.5
1585.590	5_3_1_0	Intact HMO	1417829	20.5
2354.820	11_2_1_0	Fucosylated high mannose	186081	20.7
2980.090	5_7_1_2	Fucosylated and sialylated complex	58089	20.7
750.291	2_2_0_0	HMO digest	514796	20.8
1115.420	3_3_0_0	HMO digest or complex/hybrid	348468	20.8
1261.480	3_3_1_0	N-glycan digest	3131834	20.8
1318.500	3_4_0_0	Complex	272553	20.8
1480.550	4_4_0_0	Complex/hybrid	430320	20.8
1382.510	5_2_1_0	Intact HMO	351419	20.9
1934.720	5_4_2_0	Fucosylated complex/hybrid	356331	20.9
1714.630	4_3_1_1	Fucosylated and sialylated complex	757691	21.0
1788.670	5_4_1_0	HMO digest or fucosylated complex/hybrid	80219	21.0
2957.100	7_6_4_0	Fucosylated complex	152530	21.1
1480.550	4_4_0_0	Complex/Hybrid	673670	21.2
1917.710	4_4_1_1	Fucosylated and sialylated complex	67225	21.2
1439.530	5_3_0_0	Intact HMO	373534	21.4
1480.550	4_4_0_0	Complex/Hybrid	1074868	21.8
1568.570	4_3_0_1	Sulfated complex	124551	21.8
1788.670	5_4_1_0	HMO digest or fucosylated complex/hybrid	154531	21.8
1934.720	5_4_2_0	Fucosylated complex/hybrid	132694	22.3
2096.780	6_4_2_0	Intact HMO or fucosylated hybrid	4336232	22.4
2137.800	5_5_2_0	Fucosylated complex/hybrid	489632	22.6
2615.980	4_6_3_1	Fucosylated and sialylated complex	49307	22.9
1480.550	4_4_0_0	Complex/Hybrid	189839	23.1
1845.690	5_5_0_0	Complex/Hybrid	429557	23.1
1950.720	6_4_1_0	Intact HMO	3551248	23.2
2022.740	5_3_2_1	Intact HMO	329446	23.3
1439.530	5_3_0_0	Intact HMO	152536	23.4
1714.630	4_3_1_1	Fucosylated and sialylated complex	382781	23.4
2210.820	6_6_0_0	Complex	194164	23.4
2323.870	4_6_1_1	Fucosylated and sialylated complex	41426	23.5
1585.590	5_3_1_0	Intact HMO	945054	23.6
1480.550	4_4_0_0	Complex/Hybrid	1275312	23.8
1804.660	6_4_0_0	Intact HMO	188455	23.8
3272.210	5_7_3_2	Fucosylated and sialylated complex	91951	23.8
2251.840	5_7_0_0	Complex	83628	24.1
1569.590	4_3_2_0	Fucosylated complex/hybrid	403151	24.2
2022.740	5_3_2_1	Intact HMO	51901	24.2
1845.690	5_5_0_0	Complex/Hybrid	352433	24.3
1876.680	5_3_1_1	Intact HMO	115578	24.3
2251.840	5_7_0_0	Complex	199570	24.3
1569.590	4_3_2_0	Fucosylated complex/hybrid	81166	24.6
1318.500	3_4_0_0	Complex	62543	25.2
1934.720	5_4_2_0	Fucosylated complex/hybrid	95705	26.1
1876.680	5_3_1_1	Intact HMO	168198	26.5
1730.620	5_3_0_1	Intact HMO or sialylated hybrid	497318	26.8
2095.760	6_4_0_1	Intact HMO or sialylated hybrid	481884	27.1
2219.860	3_7_2_0	Fucosylated complex	380620	27.8
1204.460	3_2_2_0	HMO digest	46764	28.0