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. 2016 Sep 7;6:32707. doi: 10.1038/srep32707

Figure 3. Activation of GIRK channels with ML297 applied in the preBötC region decreased rhythmic breathing and genioglossus muscle amplitude in anesthetized rats.

Figure 3

Microperfusion (a) the selective GIRK channel activator ML297 (50 μM) in the preBötC region reduced breathing rate and genioglossus muscle amplitude, but not diaphragm amplitude (b,c). Dots indicate breathing rate for each breath. ML297 (50 μM) significantly reduced breathing rate (n = 11, d), but not diaphragm muscle amplitude (f). ML297 also significantly diminished genioglossus muscle amplitude. ML297 at higher concentration (200 μM strongly reduced breathing rate and genioglossus muscle amplitude, but not diaphragm amplitude (e). *Indicate mean values significantly different from aCSF/baseline with P < 0.05. Data are indicated as means ± S.E.M and as individual values for each animal. aCSF, artificial cerebrospinal fluid. Dia, diaphragm muscle. GG, genioglossus muscle.