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. 2016 Sep 7;6:32694. doi: 10.1038/srep32694

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Square regions of interest were classified by the user as samples to train the program to segment the rest of the image, where biofilm is labelled green and the surface is labelled magenta. (b) Whole image segmented automatically from the samples given in (a). The Weka logo http://www.cs.waikato.ac.nz/ml/images/Weka (software) logo.png> is available under the Creative Commons Attribution-ShareAlike 2.5 http://creativecommons.org/licenses/by-sa/2.5/ License (http://creativecommons.org/licenses/by-sa/2.5/) for the use of the logo in this figure. Please see the terms of use on the Weka website for more information: http://www.cs.waikato.ac.nz/ml/weka/citing.html.