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. 2016 Sep 7;6:32834. doi: 10.1038/srep32834

Figure 2. TNFR2 deficiency impairs the capacity of naive CD4 T cells to expand in the Rag 1−/− mice.

Figure 2

Naïve CD4 (nCD4) cells (CD4+CD25CD45RBhi) were flow-sorted from Ly5.2 WT B6 mice (CD45.2) and TNFR2−/− mice (CD45.2+) and mixed at a 1:1 ratio. The cells (6 × 105 cells/mouse) were transferred into Rag 1−/− mice. After 4 weeks mice were sacrificed. (A) The proportion of WT CD4 T cells (CD45.2) and TNFR2−/− CD4 T cells (CD45.2+) in spleen, mesenteric LNs (mLNs), axillary/inguinal LNs (aiLNs), blood or cLP of recipient Rag 1−/− mice was analyzed by FACS, gating on live CD45+CD4+TCRβ+ cells. (A) shows the typical FACS analysis and (B) shows the summary of data (means ± SD, N = 3). Comparison (Two-way ANOVA test) of WT and TNFR2−/− CD4 cells in the same organ, ***p < 0.001. Data shown are representative of three separate experiments with similar results.