Figure 6. TNFR2 deficient naive CD4 T cells expressed markedly higher ratio of p100/p52.
Naïve CD4 cells (CD4+CD25−CD45RBhi) were flow-sorted from WT or TNFR2−/− mice. Immunoblotting analysis of p100 and p52 using whole cell lysates from naive CD4 T cells. Βeta-actin expression was used as loading control. (A) Shows a representative result of three independent experiments. (B) Shows the summary of ratio of p100/p52 (n = 3). Data shown are representative of three separate experiments with similar results. Comparison of the difference between two groups, *p < 0.05 (two-tailed Student t Test).