Table 1. Real-time PCR primer and size of the amplification products of the target and housekeeping genes.

Gene	Forward primer, 5′-3′	Reverse primer, 5′-3′	Product, bp
NUCB2	CCAGACACGGGACTTTATTATG	CCGCTCCTTATCTCCTCTATGT	122
FSHβ	AGACCAAACACCCAGAAAG	TCACTATCACACTTGCCACA	140
GnRH	GCCGCTGTTGTTCTGTTGAC	CTGGGGTTCTGCCATTTGA	153
Kiss-1	TGCTGCTTCTCCTCTGTGTG	CCAGGCATTAACGAGTTCCT	117
LHβ	CATAGTCTCCTTTCCTGTGGC	CATTGGTTGAGTCCTGGGA	91
StAR	CAACTGGAAGCAACACTCTACA	ACACCTGGCACCACCTTACT	164
P450scc	AACGGCACACACAGAATCCAT	AAGAGAGTCGCTGCGTCCTTAG	128
3β-HSD	GTGTATGTAGGCAATGTGGC	ACTGGAATCAAGGTGGAGG	180
17β-HSD	AGCCAAGAAGTTAGGAGGAAAC	CACAGTTGACAGCCACATCT	125
β-actin	CCCATCTATGAGGGTTACGC	TTTAATGTCACGCACGATTTC	150
Ywhaz	TTGAGCAGAAGACGGAAGGT	GAAGCATTGGGGATCAAGAA	136