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. 2016 Sep 7;6:32755. doi: 10.1038/srep32755

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A,B) were the photos of traditional ELISA and this dual-color ELISA in detection of PSA; (C) UV-vis spectrum of this dual-color ELISA; (D) calibration curve of the traditional ELISA; (E,F) were the calibration curve of wine red section and yellow section which monitored separately by the changes of absorption value at 530 nm (ΔA₅₃₀) and absorption value at 450 nm (A₄₅₀), respectively. Error bars represent standard deviations of three replicates.