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. 2016 Sep 7;6:32771. doi: 10.1038/srep32771

Figure 3. Osteogenesis in MC3T3-E1, primary osteoblasts and mouse BMSCs expressing AMPK α1 and α2.

Figure 3

MC3T3-E1 cells were induced with osteogenic differentiation. On day 7 after induction, the expression of the osteogenesis markers Runx2 (A), Alp (B), Ibsp (C), and Ocn (D) was evaluated by qRT-PCR. On days 9 and 12 after application of BMP2 and the AMPK agonist AICAR, calcium deposition was assessed by Alizarin Red staining (E) and quantification (F). Primary osteoblasts were induced with osteogenic differentiation. On day 9 after induction, the expression of Osterix (G), Alp (H), Ibsp (I) and Ocn (J) was evaluated by qRT-PCR. On days 15 after induction, calcium deposition was assessed by Alizarin Red staining (K) and quantification (L). Mouse BMSCs were induced with osteogenic differentiation. On day 7 after induction, the expression of Runx2 (M), Alp (N), and Ocn (O) was evaluated by qRT-PCR. On days 14 after induction, calcium deposition was assessed by Alizarin Red staining (P) and quantification (Q). β-actin was used as an internal control of qRT-PCR. Data are shown as mean ± SD and are expressed as fold changes in mRNA abundance relative to LV-ctr cultures. *P < 0.05, **P < 0.01.