Table 3.
Quantitative analysis of the digestive enzymes proteome
| Enzyme | Fasting (NSAF) |
Fed (NSAF) |
Fed/Fasting | p-value |
|---|---|---|---|---|
| Astacin-like metallopeptidase 21 | 1.1 ± 0.1 | 0.06 ± 0.006 | 0.055 | 0.001 |
| Chitinase | 16.9 ± 5.2 | 1.49 ± 0.619 | 0.08 | 0.007 |
| Carboxypeptidase B 1 | 2 ± 0.6 | 0.19 ± 0.237 | 0.09 | 0.009 |
| CUB/LDL trypsin 1 | 1.2 ± 0.28 | 0.2 ± 0.088 | 0.16 | 0.01 |
| Lysosomal alpha-mannosidase | 0.3 ± 0.1 | 0.05 ± 0.018 | 0.2 | 0.03 |
| Astacin-like metallopeptidase 11 | 1.3 ± 0.47 | 0.29 ± 0.153 | 0.21 | 0.019 |
| Group XV phospholipase A2 | 0.0081 ± 0.009 | 0 | 0 | * |
| Pancreatic triacylglycerol lipase | 0.093 ± 0.059 | 0 | 0 | * |
| Triacylglycerol lipase 2 | 0.021 ± 0.024 | 0 | 0 | * |
| Triacylglycerol lipase 3 | 0.049 ± 0.055 | 0 | 0 | * |
| Alpha-galactosidase A | 0.023 ± 0.015 | 0 | 0 | * |
| Alpha-N-acetylgalactosaminidase | 0.061 ± 0.06 | 0 | 0 | * |
| Beta-galactosidase | 0.01 ± 0.0074 | 0 | 0 | * |
| Lysosomal alpha-glucosidase | 0.057 ± 0.037 | 0 | 0 | * |
| Lysosomal alpha-mannosidase | 0.0092 ± 0.0089 | 0 | 0 | * |
| Maltase-glucoamylase, intestinal | 0.044 ± 0.062 | 0 | 0 | * |
| Pancreatic alpha-amylase | 0.029 ± 0.0022 | 0 | 0 | * |
| Carboxypeptidase B 2 | 0.099 ± 0.09 | 0 | 0 | * |
| Carboxypeptidase B 3 | 0.0087 ± 0.0021 | 0 | 0 | * |
| Dipeptidyl peptidase 2 | 0.014 ± 0.013 | 0 | 0 | * |
| Glutamyl aminopeptidase | 0.064 ± 0.08 | 0 | 0 | * |
| Glutamyl aminopeptidase | 0.084 ± 0.11 | 0 | 0 | * |
| Peptidase M20 domain-containing protein 2 | 0.006 ± 0.0044 | 0 | 0 | * |
| Probable carboxypeptidase PM20D1 | 0.0086 ± 0.01 | 0 | 0 | * |
| Tripeptidyl-peptidase 2 | 0.0034 ± 0.0019 | 0 | 0 | * |
| Astacin-like metallopeptidase 10 | 0.011 ± 0.0046 | 0 | 0 | * |
| Astacin-like metallopeptidase 14 | 0.014 ± 0.0083 | 0 | 0 | * |
| Astacin-like metallopeptidase 15 | 0.037 ± 0.011 | 0 | 0 | * |
| Astacin-like metallopeptidase 17 | 0.017 ± 0.013 | 0 | 0 | * |
| Astacin-like metallopeptidase 23 | 0.076 ± 0.022 | 0 | 0 | * |
| Astacin-like metallopeptidase 7a | 0.012 ± 0.0089 | 0 | 0 | * |
| Astacin-like metallopeptidase 9 | 0.015 ± 0.022 | 0 | 0 | * |
| cathepsin L-like cysteine peptidase 4 | 0.0063 ± 0.0036 | 0 | 0 | * |
| CUB/LDL trypsin 2 | 0.034 ± 0.028 | 0 | 0 | * |
| CUB trypsin 1 | 0.072 ± 0.022 | 0 | 0 | * |
| CUB trypsin 2 | 0.0084 ± 0.0052 | 0 | 0 | * |
| CUB trypsin 4 | 0.011 ± 0.0095 | 0 | 0 | * |
The values are mean ± SD of three distinct biological replicates
Abbreviation: NSAF normalized spectral abundance factor
*p-value not calculated