Table 4.
Non-digestive proteins identified by mass spectrometry
| Protein | Digestive Fluid (%) | Fasting (%) | Fed (%) | SPCS |
|---|---|---|---|---|
| Venom peptide isomerase (heavy chain) | 0.53 | N.I | N.I | None |
| Venom allergen 5 | 0.44 | N.I | N.I | Inc |
| Venom allergen 5 | 0.22 | N.I | N.I | Inc |
| U24-ctenitoxin-Pn1a | 1.82 | 1.6 | 0.38 | 17–18 |
| U24-ctenitoxin-Pn1a | 0.11 | N.I | N.I | 22–23 |
| U24-ctenitoxin-Pn1a | 0.09 | 0.06 | 0.35 | 17–18 |
| U24-ctenitoxin-Pn1a | 0.08 | 0.2 | 0.02 | 19–20 |
| U24-ctenitoxin-Pn1a | 0.04 | 0.08 | Id | 17–18 |
| U24-ctenitoxin-Pn1a | N.I | 0.05 | 0.22 | 22–23 |
| U9-ctenitoxin-Pr1a | N.I | 0.009 | Id | Inc |
| Protease inhibitor U1-aranetoxin-Av1a | N.I | 0.41 | 0.57 | None |
| U3-aranetoxin-Ce1a | N.I | 0.06 | I | None |
| L-cystatin | 1.2 | 0.26 | N.I | 17–18 |
| Cystatin A2 | N.I | 0.7 | 1.16 | None |
| Serpin B3 | 0.06 | 0.07 | 0.03 | Inc |
| Serpin B6 | N.I | 0.03 | N.I | None |
| Leukocyte elastase inhibitor | 0.07 | N.I | N.I | Inc |
| Leukocyte elastase inhibitor | 0.003 | N.I | N.I | 21–22 |
| Alpha-1 inhibitor 3 | 0.04 | 0.008 | 0.002 | 27–28 |
| Peritrophin-1 | N.I | 0.16 | 0.74 | Inc |
| Peritrophin-48 | 0.008 | 0.04 | N.I | Inc |
| Transferrin | 0.2 | 0.09 | 0.63 | Inc |
| Soma ferritin | 0.05 | 5.1 | 2.9 | None |
| Soma ferritin | N.I | 0.21 | 0.08 | Inc |
| sum of leucine-rich repeat-containing proteins | 5.9 | a | a | a |
The values are the means of the percentages from the normalized spectral abundance factor to each sample. The data from midgut diverticula are from three distinct biological replicates and only proteins identified in at least two samples were used for quantitative analysis. Digestive juice NSAF is the sum of all different periods of feeding (fasting, 1, 3, 9, 25, 30 and 48 h
Abbreviations: SPCS signal peptide cleavage site, Inc incomplete N-terminal sequence, N.I protein not identified, Id identified in only one replicate
aprotein not searched in that sample or SPCS not analyzed