Table 1. Crystal and data-collection parameters.
| Macromolecule | Glucose isomerase | Ultralente insulin | Thermolysin | LUX–DNA | Urease |
|---|---|---|---|---|---|
| Space group | I222 | H3 | P6122 | P1 | P6322 |
| Unit-cell parameters (Å, °) | a = 93.1, b = 99.5, c = 102.4 | a = 82.2, b = 82.2, c = 33.8 | a = 92.9, b = 92.9, c = 129.5 | a = 32.9, b = 70.9, c = 67.0, α = 103.9, β = 92.4, γ = 91.0 | a = b = 132.43, c = 190.6, |
| Beamline | ID23-EH2, ESRF | ID23-EH2, ESRF | ID29, ESRF | ID23-EH2, ESRF | P13, PETRA III |
| Wavelength (Å) | 0.8731 | 0.8731 | 1.280 | 0.8731 | 2.0664 |
| Beam size (H × V or diameter) (µm) | 9 × 5 | 9 × 5 | 10 × 10 | 9 × 5 | 30 |
| Crystal size range (µm) | 10 × 10 × 10–30 × 30 × 30 | 5 × 5 × 5–15 × 15 × 15 | 20 × 20 × 100 | 25 × 5 × 5–100 × 5 × 5 | 20 × 20 × 40–20 × 20 × 70 |
| Photon flux (photons s−1) | 1.6 × 1011 | 7.0 × 1010 | 4.1 × 1011, 8.4 × 1011 | 4.4 × 1010 | 3.4 × 1011 |
| Exposure per image (s) | 0.1 | 0.25 | 0.037 | 0.1 | 0.04 |
| No. of images per sub-data set | 140 | 100 | 100 | 100 | 300 |
| Dose per sub-data set (average diffraction-weighted dose) (MGy) | 6.0–7.3 | 5.3–7.5 | 3.0–6.2 | 1.67 | 0.48 |
| Dose per sub-data set (average dose exposed region) (MGy) | 1.1–7.8 | 2.96–10.5 | 4.2–8.7 | 0.20–0.63 | 0.82 |
| Oscillation range (°) | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| Total angular range per sub-data set (°) | 14 | 10 | 10 | 10 | 30 |