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. 2016 Aug 31;7:12639. doi: 10.1038/ncomms12639

Figure 5. Ectopic March1 expression is sufficient to impair hepatic insulin action in mice.

Figure 5

Male 12-week-old C57BL/6 J mice were injected intravenously with AAV 4 weeks before study and fed regular chow. (a) Immunoblot analysis of GFP and March1 protein expression after AAV treatment. (b) qRT-PCR analysis of hepatic mRNA March1 expression after AAV treatment. (c) Plasma glucose and glucose infusion rates during hyperinsulinemic-euglycemic clamp studies. (d) Mean steady-state glucose infusion rates required to maintain euglycemia during the clamp. (e) EGP during the basal period and during the steady-state period of the clamp. (f) EGP suppression. Data are mean±s.e.m. In all panels, *P<0.05, ***P<0.0005. n=7-8 mice per group; comparisons by two-tailed unpaired t-test. qRT-PCR, quantitative real-time PCR.