Figure 4. Notch2-deficient Ly6C^hi monocytes show impaired conversion potential in vivo.

Flow-cytometry based quantification of the fraction of (a) apoptotic cells (AnnexinV⁺7-AAD⁻) or (b) dead cells (7-AAD⁺) in monocyte subsets. Data are pooled from three-independent experiments (n=8/12). (c) Flow cytometry four days after adoptive transfer of BM Ly6C^hi monocytes from control or Notch2-deficient CD45.2⁺GFP⁺ donors into CD45.1⁺ congenic recipients. Transferred cells are shown in black and for comparison, recipient CD45.1⁺ (first row), CD45.1⁺CD11b⁺ (second row), CD45.1⁺CD11b⁺Ly6C^hi (third row) cells or CD45.1⁺CD11b⁺Ly6C^hiF4/80^lo monocytes (fourth and fifth rows) are depicted in blue. (d) Frequency of donor-derived Ly6C^lo monocytes (left) or Ly6C^hi monocytes (right) in the CD11b⁺GFP⁺CD45.2⁺ gate after adoptive transfer of Ly6C^hi monocytes. Data are pooled from four-independent experiments (n=5/7). (a,b,d) *P<0.05, **P<0.01, ***P<0.001; Student's t-test. Error bars represent s.e.m.