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. 2016 Aug 31;7:12597. doi: 10.1038/ncomms12597

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Copyright © 2016, The Author(s)

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(a,b) Kinetics of monocyte conversion in the presence of Notch ligand DLL1 in vitro. Representative flow cytometry plots (a) and frequency (b) of Ly6C^lo monocyte-like cells (CD11b⁺GFP⁺Ly6C^lo/−CD11c^loCD43⁺MHC-II^lo/−) calculated from live CD11b⁺GFP⁺ cells are shown (n=3). (c) Gene expression analysis in in vitro cultures from sorted BM Ly6C^hi monocytes. Change relative to expression of genes in control cultures is shown (n=6). (d,e) DLL1 specifically induces conversion in vitro. Representative flow cytometry plot (d) and frequency (e) of Ly6C^lo monocyte-like cells (similar to Fig. 5a,b) are shown after culture of Ly6C^hi monocytes alone or in the presence of control, JAG1 or DLL1 ligands. (b,c,e) *P<0.05, **P<0.01, ***P<0.001; one-way ANOVA with Bonferroni's multiple comparison test. Error bars represent s.e.m.