Table 4.

Three-way ANOVA of the effects of time, temperature and pH on the bactericidal activity of coelomocytes collected from Heliocidaris erythrogramma exposed to temperature and pH treatments for 1, 15 and 30 days. Bactericidal activity was measured after (a) 0 (b) 24 and (c) 48 h after bacterial suspensions and coelomic fluid were combined (n = 5 per treatment). Significant factors (p < 0.05) are indicated in italics.

measurement	factor	d.f.	MS	F	p-value	Tukey's HSD
(a) bacterial survival—0 h	time	2	31.22	0.50	0.608
	temperature	1	0.27	0.00	0.948
	pH	1	72.6	1.17	0.285
	time : temperature	2	3.32	0.05	0.948
	time : pH	2	45.15	0.73	0.488
	temperature : pH	1	15.00	0.24	0.625
	time : temperature : pH	2	13.65	0.22	0.803
	residuals	48	62.06
(b) bacterial survival—24 h	time	2	206 552	5.93	0.005	D30 > D1 = D15
	temperature	1	622 405	17.87	<0.0001	23°C > 17°C
	pH	1	420 509	12.07	0.001	7.6 > 8.15
	time : temperature	2	3443	0.10	0.906
	time : pH	2	58 958	1.69	0.195
	temperature : pH	1	23	0.00	0.98
	time : temperature : pH	2	276 014	7.92	0.001	D1 23°C/7.6 > D1 17°C/8.15; D30 23°C/8.15 > D30 17°C/8.15
	residuals	48	34 835
(c) bacterial survival—48 h	time	2	6 473 404	6.18	0.004	D30 > D1; D30 > D15
	temperature	1	8 836 611	8.44	0.006	23°C > 17°C
	pH	1	12 514 493	11.95	0.001	7.6 > 8.15
	time : temperature	2	2 243 521	2.14	0.129
	time : pH	2	3 225 822	3.08	0.055
	temperature : pH	1	3 630 960	3.47	0.069
	time : temperature : pH	2	566 451	0.54	0.586	D30 23°C/7.6 > D30 17°C/8.15
	residuals	48	1 047 356