Experimental design of RNAseq studies to identify the transcriptional basis of diapause in Ae. albopictus during the stages of diapause induction, preparation, and developmental arrest (Huang et al. 2015; Poelchau et al. 2011, 2013a, 2013b). The transcriptomes of mosquitoes reared under diapause-inducing and nondiapause inducing photoperiods are compared at different developmental stages (adult females, stage V oocytes, embryos 72–78 h post oviposition, embryos 135–141 h post oviposition, and eggs 11, 21, and 40 d post oviposition).