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. Author manuscript; available in PMC: 2016 Sep 7.
Published in final edited form as: Methods Mol Biol. 2016;1338:157–177. doi: 10.1007/978-1-4939-2932-0_13

Figure 3. Variability within the uninjected control samples reduces the sensitivity of HRMA analysis of gene editing events.

Figure 3

The HRMA melt profiles (top) show excessive variability between replicate control DNAs (n=6), compared to those (bottom) that show relatively little variation (n=7).