Figure 6.

GREM2 Sensitivity of BMP4 Target Genes in human fetal ovarian somatic cells. FT3330 cells were serum-starved overnight and treated with ± 100 ng/ml GREM2, ± 50 ng/ml BMP4 (n = 4 replicate wells for each treatment) for 24 h before RNA extraction and qRT-PCR analysis of key BMP4 targets, with normalisation to RPL32 transcript levels. Columns represent mean ± sem for each treatment (white = vehicle, red = GREM2, blue = BMP4, purple = BMP4 + GREM2). Differences in expression levels between treatments were analysed by ANOVA with Tukey's multiple comparisons post-hoc tests and significant differences (P ≤ 0.05 or less, as indicated in the main text) between treatments are indicated by different letters above the columns.