Table II.
Treatment | Source | Cat. No. | Vehicle | Stock Conc. | Working Conc. |
---|---|---|---|---|---|
Recombinant Human BMP4 | Life Technologies (Paisley, UK) | PHC-9534 | 4 mM HCl/ 0.1% BSA in PBS | 10 μg/ml | 1–100 ng/ml as indicated in text |
Recombinant Human BMP2 | Life Technologies (Paisley, UK) | PHC-7145 | 4 mM HCl/ 0.1% BSA in PBS | 100 μg/ml | 1–100 ng/ml as indicated in text |
Recombinant Human Gremlin, CF | R&D Systems (Abingdon, UK) | 5190-GR-050 | PBS | 200 μg/ml | 1 μg/ml |
Recombinant Human PRDC/GREM2, CF | R&D Systems (Abingdon, UK) | 8436-PR-050 | 4 mM HCl in PBS | 500 μg/ml | 100 ng/ml |