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. 2016 Aug 19;18(17):4288–4291. doi: 10.1021/acs.orglett.6b02033

Figure 2.

Figure 2

Investigation of the KS1–ACP1–KS2 domain of LnmI PKS module 3 at the LnmI NRPS–PKS interface for LNM biosynthesis by site-directed mutagenesis in vivo. (A) HPLC analysis of LNM production from KS1 or KS2 mutants as well as the complementation strains: I, SB3036 (C2090A); II, SB3037 (H2959A); III, SB3038 (C2824A); IV, SB3045; V, SB3046; VI, SB3047; VII, S. atroolivaceus S-140 wild-type; VIII, LNM standard (●). (B) HPLC analysis of LNM production from the ACP1 mutants: I, SB3039; II, SB3040; III, SB3041; IV, SB3042; V, SB3043; VI, SB3044; VII, S. atroolivaceus S-140 wild-type; VIII, LNM standard (●).