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Validation of the direct CTLA4-CD28 fusion cases. Direct fusion of 3rd exon of CTLA4 and 4th exon of CD28 were confirmed by PCR using primers from intron2 of CTLA4 and 3’ region of CD28 using genomic DNA from FFPE samples. Arrows indicate the approximate positions of oligonucleotide primers on the CTLA4-CD28 fusion gene. PCR products were validated by Sanger sequencing. DNA from normal blood was used as control and NTC indicates the no template control.
