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. 2016 Jan 25;5(3):275–282. doi: 10.1080/21623945.2015.1134402

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — Both undifferentiated ASC and ASC-derived adipocytes (adipose cells, AC) polarize T cells toward the Th17 lineage and down-regulate Th1 cytokines. ASC differentiation was carried out for 8 d or 14 d as indicated. Cell culture supernatants of MNC co-cultured or not with undifferentiated ASC (light gray bars), differentiated adipocytes at day 8 (gray bars) or at day 14 (black bars), and activated by PHA or not, were analyzed by ELISA for the secretion of IL-17A (A) and TNFα (B). Co-cultures were in a 1:5 ratio (20,000 ASC/adipocytes for 100,000 MNC). Error bars represent standard deviations from n ≥ 3 independent experiments. ***p < 0.001 as tested by one-way ANOVA followed by Bonferroni's multiple comparison test. n.s not statistically significant for each combinatorial comparisons within the 3 bars.