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. 2016 Jan 25;5(3):275–282. doi: 10.1080/21623945.2015.1134402

Figure 4.

Figure 4.

ASC-derived adipocytes from lean donors induce IL-17A secretion in MNC-adipocytes co-cultures. ASC from obese (dark gray bars) or lean (light gray bars) donors were differentiated for 8 d. Cell culture supernatants of MNC co-cultured or not with differentiated adipocytes were activated by PHA or not, and analyzed by ELISA for the secretion of IL-17A. Co-cultures were in a 1:5 ratio (20,000 ASC/adipocytes for 100,000 MNC). Error bars represent standard deviations from n ≥ 3 independent experiments. ***p < 0.001; as tested by one-way ANOVA followed by Bonferroni's multiple comparison test. n.s not statistically significant.