Table 3.

Oligonucleotides to construct and validate MSE deletion.

Purpose	ORC1 50:50-U2	ORC1 D2-50
MSE deletion	5′-TAAGTGCATGGTATGGAGTGTATAATGGTTTATAATTTCCCCTAAGATAAATGTTCTCCCAAAAATTTACCAAGAAAAAAAATTAAGAATACTACACACGTACGCTGCAGGTCGAC-3′	5′-TGTGTAGTATTCTTAATTTTTTTTCTTGGTAAATTTTTGGGAGAACATTTATCGATGAATTCGAGCTCG-3′
	Forward primer	Reverse primer
Validation of MSEΔORC1	5′-TGGTATGGAGTGTATAATGG-3′	5′-CCCAAGCATCAATTGTGTAG-3′