Figure 3.

Stem loop structure of SL3–10(−) predicted at nucleotide positions 219–240 of M segment -RNA. (A) Secondary structures were predicted using the M nucleotide sequence of England/195/2009(H1N1) (accession number GQ166660) at temperature setting of 37°C. The background red color of each base represents nucleotide identities of > 98% among 1,884 sequences. Third codon positions are circled in blue. The red arrows and nucleotide sequences indicate the position and synonymous mutations, which were introduced to produce SL3–10 mutant. (B) The number (percentage) of sequences that were predicted to form base-paring (BP) at the stem positions of SL3–10(−) among 1,884 sequences. (C) Secondary structure predicted at positions 219–240 of SL3–10 M sequence. Bases with synonymous mutations are circled in red.