Table 3.
Oligonucleotides used in this study. Restriction sites are underlined.
| Name | 5′–3′ DNA sequence | Cloning site |
|---|---|---|
| ivTr_6C_fw | TCTAGATAATACGACTCACTATAGGCAAGGCCCCGATATACAGTG, T7 promoter sequence used is given in bold | XbaI |
| ivTr_6C_rv | AGATTATAAGGGACGGCCCGCGCCT | PsiI |
| 6C_P1fw | TTTAAGTCGACAGTGACCACCGAATGCATGG | SalI |
| 6C_P2rv | CCCATCCACTAAACTTAAACAGAATAACACGAATACTTAACAGTGTGG, overlap with 6C_P3fw is given in bold | – |
| 6C_P3fw | TGTTTAAGTTTAGTGGATGGGTTTCTAAACTAGTGCCCTTAGCAG, overlap with 6C_P2rv is given in bold | – |
| 6C_P4rv | AATTTGAATTCGTTGTTTGTCATCTCTGCCTAAAGC | EcoRI |
| nP_6Cfw | TTTAAGGATCCTGGCAACTTTCCAAGACTGAG | BamHI |
| nP_6Crv | AAATTGTCGACCACAGAAAATCTGCTAAGGGCA | SalI |
| nP_6Cfw2 | AACTGCAGTTACCAATGAGATTGAAG | PstI |
| nP_6Crv2 | TAGGATCCAGGCCACACTGTATATCG | BamHI |
| SigAF | GCGAATTCATCCTCAGCATCACTC | EcoRI |
| SigAR | TGTCTAGACGAACCAAAGCAACAG | XbaI |
| SigBF | AGGAATTCGTTGAACCTCTTGAAC | EcoRI |
| SigBR | GCTCTAGAACTCGCCGGTAAAATA | XbaI |
| SigHF | AAGAATTCTGCAGATAGTCAACACGCATTTTCGAAAGGGGC | EcoRI |
| SigHR | AATCTAGACGAGTCGCTGCGGTTGAGA | XbaI |
| SigMF | AAGAGCTCTGCAGGCCCATACTAAGCCGCATAA | SacI |
| SigMR | AATCTAGATCAAAGTAGTAAACGTAAAAGTGC | XbaI |
| SigEF | AAGAATTCGTCGACAGGATGAGGATCGTTTCGCATGAAAAAGAAGTCCCGAGATGACGCACCCG | EcoRI |
| SigER | AATCTAGAAAGTTCCCTGTCCGCACCAC | XbaI |
| P6C_IVTfw | CTGAGAATTCAACTAAAGTTACCAATGAG | EcoRI |
| P6C_IVTrv | TCGGAAGCTTGCACCTGAAATTATG | HindIII |
| NB_fw | TGGCGTTTGATATGCCATAATTTCAGGTGC | – |
| NB_rv | ACAGAAAATCTGCTAAGGGCACTAGTTTAG | – |
| P6C_fw | CGTAGGCGTAGGTGGAACTC | – |
| P6C_rv | GAGGAGTCGTCGATGTGGAGACCCACACTGTATATCGGGGCCTT, overlapping Biotin_rv sequence below is given in bold | – |
| Biotin_rv | BITEG-GAGGAGTCGTCGATGTGGAGACC | – |
| P6C_rv2 | GCACCTGAAATTATGGCATATC | – |
| PaceAB_fw | TCAAGTCGACTTCCTTAAGTGCTGATTCG | – |
| PaceAB_rv | CGGGATCCCGCTCCTTTTAAAGCATGGG | – |
| Ppgi_fw | CCTACCTTCTCGATCCCTTCTC | – |
| Ppgi_rv | CCTGGGTGGTCGAAATGTC | – |
| LexA_F1_rv | CACGAATACTTAACAGTGTGGC | – |
| LexA_F2_fw | GGAAGTGTCATTTGCCACACTG | – |
| LexA_F2_rv | CACTTTCGAATGCAGAGGCACAG | – |
| LexA_OP1fw | CGTGTTATTCGAATAAATAATCGGGTTTGATGpredicted LexA operator sequence(+) is given in bold | – |
| LexA_OP1rv | CATCAAACCCGATTATTTATTCGAATAACACGpredicted LexA operator sequence(−) is given in bold | – |
| LexA_OP2fw | TTGCCACACTGTTAAGTATTCGTGTTATTCoverlap with predicted LexA operator sequence(+) is given in bold | – |
| LexA_OP2rv | GAATAACACGAATACTTAACAGTGTGGCAAoverlap with predicted LexA operator sequence(−) is given in bold | – |
| LexA_OP3fw | GGTTTGATGTTGTCTTGTGGCGTTTGATATGoverlap with predicted LexA operator sequence(+) is given in bold | – |
| LexA_OP3rv | CATATCAAACGCCACAAGACAACATCAAACCoverlap with predicted LexA operator sequence(−) is given in bold | – |