Figure 5.

DNMT1 mutants. (A) Representative images from fluorescence aggresomes marker staining of HEK293 cells transfected with either enhanced GFP-wild type DNMT1 or various mutant enhanced GFP-DNMT1. Wild-type DNMT1 stays in the nucleus and binds to heterochromatin whereas mutant DNMT1 aggregates in cytosol overlaid with red fluorescence aggresome markers. Scale bars = 5 µm. (B) Quantification of GFP-DNMT1 proteins in HEK293 cells transfected with eGFP-wild type DNMT1, or various mutant enhanced GFP-DNMT1. All mutant DNMT1 proteins showed degradation comparing to wild-type DNMT protein. Data are derived from three independent experiments, asterisk represents there is statistically significant change between wild-type and mutant proteins.