Mice were treated with antibiotic (AB) mix or vehicle (VEH) by gavage for 11 days. The panels show the expression of tight junction protein 1 (TJP1), claudin 5 (CLDN5) and occludin (OCLN) mRNA in the medial prefrontal cortex (A), hippocampus (B), amygdala (C), and hypothalamus (D). mRNA expression is expressed as fold change relative to VEH-treated mice. Values represent means + SEM, n=10-12; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared to VEH-treated mice, t-test.