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. 2016 Jul 12;67(17):5009–5027. doi: 10.1093/jxb/erw266

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Overexpression of CRK5, but not its mutated form CRK5 ^K372E, results in an ABA-hypersensitive phenotype in ABA-induced inhibition of cotyledon greening. (A) Cotyledon greening of wild-type Col-0, CRK5-transgenic lines OE-1 and OE-2, and CRK5 ^K372E-transgenic lines CRK5^K372EOE-1 and CRK5^K372EOE-2 in ABA-free (0 μM, top) or (±)ABA-containing (0.5 μM, bottom) MS medium. (B) Percentages of green cotyledons of the different genotypes as described in (A). Green cotyledons were scored 5 days after stratification. Values are the mean±SE of three biological determinations, and different letters represent significant differences at P<0.05 (Duncan’s multiple range test).