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. 2016 Jul 12;67(17):5009–5027. doi: 10.1093/jxb/erw266

Fig. 3.

Fig. 3.

Overexpression of CRK5, but not CRK5 K372E, results in ABA hypersensitivity in stomatal movement and increases plant drought tolerance. (A) ABA-induced inhibition of stomatal opening (left) and promotion of stomatal closure (right) in wild-type Col-0 plants, CRK5-transgenic lines OE-1 and OE-2, and CRK5 K372E-transgenic lines CRK5K372EOE-1 and CRK5K372EOE-2. The experiments were repeated five times with similar results. The values are the mean±SE from 60 stomata for each time point, and different letters represent significant differences at P<0.05 (Duncan’s multiple range test). (B) Test of drought tolerance of the different genotypes described above. Plants were well watered (control, ‘Well water’) or drought stressed by withholding water (‘Drought’) for 16 d (D) and then re-watered (‘Rewater’). The experiments were repeated five times, and at least 30 plants per individual line were used for each experiment. (C) Survival rates of the plants described in (B). The values are the mean±SE of three biological determinations, and different letters represent significant differences at P<0.05 (Duncan’s multiple range test).