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. 2016 Jul 12;67(17):5009–5027. doi: 10.1093/jxb/erw266

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Subcellular localization of CRK5 protein and expression profile of CRK5 gene. (A) CRK5 is localized to plasma membrane. The Col-0 plants were transformed with the construct carrying CRK5-GFP or empty GFP, respectively, driven by CaMV 35S promoter, and the roots of transgenic plants were investigated by a confocal laser scanning microscope. (a) CRK5–GFP localization in the mature root zone. (b) FM4-64 staining of the CRK5-GFP-transgenic plant in the mature root zone. (c) The corresponding bright field of (a) and (b). (d) Merged imagine of (a), (b) and (c). (e) Empty GFP localization in the mature root zone. (f) FM4-64 staining of GFP-transgenic plants in the mature root zone. (g) The corresponding bright field (e) and (f). (h) Merged imagine of (e), (f) and (g). Bars=20 μm. (B) Expression of the CRK5-promoter–GUS in transgenic lines. (a) Dry seed. (b) Young seedling 48h after stratification. (c) Young seedling 72h after stratification. (d) Young seedling 14 d after stratification. (e) Young seedling 21 d after stratification. (f) Rosette leaves and stomata (shown at bottom, right). (g) Flower. (h) Silique. (C) Relative expression levels of CRK5 in different tissues/organs determined by real-time PCR analysis.