Fig. 7.
Expression of some ABA-responsive genes in CRK5-transgenic line OE-1 and CRK5 K372E-transgenic line CRK5K372EOE-1. The seeds were germinated and grown on ABA-free (–ABA) or 0.5 μM-ABA-containing (+ABA) MS medium for 4 days before sampling for RNA extraction. Transcription levels of these genes were assayed by real-time PCR. Expression level of each gene is normalized to that of Actin2/8, and the relative expression level of each gene is normalized relative to the level of this gene of the wild-type Col grown in ABA-free medium, which is taken as 1. Values are the mean±SE of three independent biological determinations, and different letters represent significant differences at P<0.05 (Duncan’s multiple range test).