Fig. 6.

Comparisons between in vitro (filled symbols) and in vivo (open symbols) values of the Rubisco specificity factor for CO₂/O₂ (S_c/o) (A, B) and the Michaelis–Menten constants for CO₂ (K_c) (C, D) and O₂ (K_o) (E) at a range of temperatures for species with available data. Equation 12 was used to derive estimates for these parameters at discrete temperatures from data in Tables 2 and 4. The in vitro liquid-phase data were standardized for a common set of physico-chemical characteristics of CO₂ and O₂ as explained in Figs 1 and 2, while the in vivo gas-phase data were converted to the liquid phase as explained in Fig. 5 (Table 1 for pertinent Henry’s law constant to convert between liquid- and gas-phase equivalents). S_c/o data for Spinacia oleracea are averages for the studies Jordan and Ogren (1984), Uemura et al. (1997), Zhu et al. (1998) and Yamori et al. (2006). S_c/o data for Triticum aestivum are averages for studies Haslam et al. (2005) and Hermida-Carrera et al. (2016).